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KMID : 0950020050310020001
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Journal of Health Science & Medical Technology
2005 Volume.31 No. 2 p.1 ~ p.5
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Purification of Structured Lipid using Supercritical Fluid
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Kim In-Hwan
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Abstract
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Supercritical fluid system was applied to purify structured lipid from reaction mixture obtained from lipase-catalyzed acidolysis. The structured lipid was synthesized by acidolysis of olive oil and caprylic acid using Lipozyme RM IM from Rhizomucor miehei as a biocatalyst. Originally oleic acid was the predominant fatty acid on olive oil, composing over 80%. After acidolysis reaction, however, caprylic acid as well as oleic acid became the major fatty acids in structured lipid. After 24 h incubation, 21.0% of caprylic acid was incorporated into olive oil by Lipozyme IM. In order to remove free fatty acid from reaction mixture, supercritical carbon dioxide was used. Extraction process by supercritical carbon dioxide was carried out in the pressure range of 2000 to 3500 psi at 60 ℃. Finally, 2000 psi was an optimum pressure to purify structured lipid.
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KEYWORD
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Acidolysis, Caprylic acid, Lipase, Olive oil, Structured lipid, Supercritical fluid
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